| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| 3xFLAG | — | three tandem FLAG(R) epitope tags, foll… | 521..593 | Fwd | |
|
DNA Sequence
GACTACAAGGATGACGATGACAAGGACTACAAGGATGACGATGACAAGGACTACAAGGATGACGATGACAAG
Protein Sequence
DYKDDDDKDYKDDDDKDYKDDDDK
|
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| 10xHis | — | 10xHis affinity tag | 1562..1592 | Fwd | |
|
DNA Sequence
CACCACCACCACCACCACCACCACCACCAT
Protein Sequence
HHHHHHHHHH
|
|||||
| GmR | aacC1 | gentamycin acetyltransferase | 3352..3886 | Rev | |
|
DNA Sequence
ATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGCGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAA
Protein Sequence
MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALDLFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRSEIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIREEVMHFDIDPSTAT
|
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| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| tight TRE promoter | — | — | 197..512 | Fwd | |
|
DNA Sequence
GAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGTTTATCCCTATCAGTGATAGAGAACGTATGTCGAGTTTACTCCCTATCAGTGATAGAGAACGTATGTCGAGGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCC
|
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| Pc promoter | intI1 (promoter lies within the coding sequence) | — | 4074..4103 | Rev | |
|
DNA Sequence
TTGACATAAGCCTGTTCGGTTCGTAAACT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| attL1 | mutant version of attL | — | 66..166 | Fwd | |
|
DNA Sequence
CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT
|
|||||
| tet operator | tetO | — | 205..224 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 241..260 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 276..295 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 312..331 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 348..367 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 383..402 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| tet operator | tetO | — | 419..438 | Fwd | |
|
DNA Sequence
TCCCTATCAGTGATAGAGA
|
|||||
| attL2 | mutant version of attL | — | 2470..2570 | Rev | |
|
DNA Sequence
CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTCTTATAATGCCAACTTTGTACAAGAAAGCTGGGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 4759..5348 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| rrnB T2 terminator | — | — | 0..5701 | Fwd | |
|
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT
|
|||||
| rrnB T1 terminator | Escherichia coli rrnB | — | 5512..5599 | Fwd | |
|
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT
|
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