pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>

Plasmid Information

Purpose

Histidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

GLT1 GAL1p 24xPP7 V3

Available Since

Aug. 8, 2016

Availability

Available

Length

985 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
SNAC tag	—	tag for protein cleavage using Ni2+ ion…	386..401	Fwd
DNA Sequence GGTTCGCACCATTGG Protein Sequence GSHHW

terminator 1

Label	Gene	Product	Position	Strand
TEF terminator	—	—	694..892	Fwd
DNA Sequence ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG