His-TEV-U_(+36GFP)-Cre

71750 Synthetic Bacterial Expression

Plasmid Information

Purpose
His-TEV-U+36GFP-Cre for E.Coli expression
Depositor
David Liu
Article
Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084
Insert
U_(pos36GFP)-Cre
Tags
6X His and TEV protease
Available Since
Jan. 25, 2016
Availability
Available

Sequence Information

Length
818 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 24..42 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 42..63 Fwd
DNA Sequence
GAAAACCTGTACTTCCAGGGC
Protein Sequence
ENLYFQG