pET28a-HsSENP3(307-574)

71481 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).
Depositor
Jorge Eduardo Azevedo
Article
Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
Insert
SUMO1/sentrin specific peptidase 3 (SENP3 Human)
Tags
Hexahistidine
Mutation
Deleted amino acids 1-306 (catalytic domain only)
Promoter
T7
Available Since
Dec. 11, 2015
Availability
Available

Sequence Information

Length
928 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 43..61 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 70..88 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 907..925 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH