pET28a-HsSENP7

71470 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).
Depositor
Jorge Eduardo Azevedo
Article
Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
Insert
SUMO1/sentrin specific peptidase 7 (SENP7 Human)
Tags
Hexahistidine
Mutation
Gene optimised for E. coli expression (Genscript)
Promoter
T7
Available Since
Jan. 19, 2016
Availability
Available

Sequence Information

Length
842 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 43..61 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 70..88 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS