pET151-hUCH37 (ISF1)(M148D/F149D)

71388 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of hUCH37 (M148D/F149D) in E.coli
Depositor
Chris Hill
Article
VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1
Insert
hUCH37 (ISF1) M148D/F149D (UCHL5 Human)
Tags
HIS-TEV sequence
Mutation
changed methionine 148 to aspartate, phenylalanin…
Promoter
T7
Available Since
Dec. 21, 2015
Availability
Available

Sequence Information

Length
728 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
6xHis 6xHis affinity tag 18..36 Fwd
DNA Sequence
CATCATCACCATCACCAT
Protein Sequence
HHHHHH
V5 tag epitope tag from simian virus 5 36..78 Fwd
DNA Sequence
GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG
Protein Sequence
GKPIPNPLLGLDST
TEV site tobacco etch virus (TEV) protease recog… 78..99 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGA
Protein Sequence
ENLYFQG