pCASCADE-gltA1-gltA2

71348 Synthetic Bacterial Expression

Plasmid Information

Purpose
gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction
Depositor
Michael Lynch
Article
Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.
Insert
gltA1-gltA2 guide array
Promoter
E . coli ugpB gene promoter, low phosphate induct…
Available Since
Jan. 31, 2022
Availability
Available

Sequence Information

Length
859 bp
Topology
linear
Molecule Type
genomic DNA

Features

terminator 2
Label Gene Product Position Strand
T7Te terminator 411..439 Rev
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG
rrnB T1 terminator Escherichia coli rrnB 454..526 Rev
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC