pSMART-Ala2

71326 Synthetic Bacterial Expression

Plasmid Information

Purpose
NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression
Depositor
Michael Lynch
Article
Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.
Insert
NADPH Alanine Dehydrogenase (ald Synthetic)
Mutation
D196A, L197R
Promoter
insulated waaHp from E. coli
Available Since
Jan. 31, 2022
Availability
Available

Sequence Information

Length
871 bp
Topology
linear
Molecule Type
genomic DNA

Features

terminator 3
Label Gene Product Position Strand
T3Te terminator 14..44 Rev
DNA Sequence
GGCTCACCTTCACGGGTGGGCCTTTCTTCG
rrnB T1 terminator Escherichia coli rrnB 127..199 Fwd
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTC
T7Te terminator 214..242 Fwd
DNA Sequence
GGCTCACCTTCGGGTGGGCCTTTCTGCG