pRS416-Gal4-dCas9-VP64

71128 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
Depositor
Ronald Davis
Article
Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
Insert
Gal4-dCas9-VP64
Tags
NLS n-terminal, N terminal Gal4 Activator domain,…
Promoter
Tef1
Available Since
March 7, 2016
Availability
Available

Sequence Information

Length
775 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
VP64 — tetrameric repeat of the minimal activa… 0..150 Rev
DNA Sequence
GACGCTTTGGACGACTTCGACTTGGATATGCTGGGTTCTGATGCGCTAGATGACTTTGACCTCGACATGCTTGGAAGTGACGCCTTAGATGATTTTGACCTGGATATGCTTGGATCAGACGCTCTGGACGATTTCGACTTAGACATGCTT
Protein Sequence
DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDML