pAG424-10X-tRNA

70125 Saccharomyces cerevisiae Yeast Expression

Plasmid Information

Purpose
Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns
Depositor
Jay Hesselberth
Article
Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126
Insert
10X-tRNA block
Promoter
None – SUP4 pol III promoter provided by insert
Available Since
April 21, 2017
Availability
Available

Sequence Information

Length
975 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

protein_bind 1
Label Gene Product Position Strand
attB2 mutant version of attB — 39..64 Fwd
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT
terminator 1
Label Gene Product Position Strand
RPR1 terminator — — 72..135 Rev
DNA Sequence
CCATATCCAACTTCCAATTTAATCTTTCTTTTTTAATTTTCACTTATTTGCGATACAGAAAGA