pET11d-FHis-DND1-Y102A

70082 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.
Depositor
Thomas Tuschl
Article
Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d
Insert
Dnd1 (DND1 Human)
Tags
6xHIS and FLAG
Mutation
Y102A. Codon optimized for bacterial expression
Available Since
Nov. 22, 2017
Availability
Available

Sequence Information

Length
700 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
FLAG FLAG(R) epitope tag, followed by an ent… 34..58 Fwd
DNA Sequence
GACTACAAAGACGATGACGATAAA
Protein Sequence
DYKDDDDK
6xHis 6xHis affinity tag 82..100 Fwd
DNA Sequence
CATCATCACCATCATCAC
Protein Sequence
HHHHHH