p5E-mfap4 — PlasmidDB

Plasmid Information

Purpose

5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter

Available Since

Nov. 17, 2015

Availability

Available

Sequence Information

Length

1,078 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	118..137	Fwd
DNA Sequence TAATACGACTCACTATAGG

protein_bind 1

Label	Gene	Product	Position	Strand
attL4	mutant version of attL	—	21..117	Fwd
DNA Sequence AAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATAAGCAATGCTTTTTTATAATGCCAACTTTGTATAGAAAAGTTG