5C7J

69965 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression for structure determination; may not be full ORF
Depositor
Cheryl Arrowsmith
Insert
NEDD4:SDC094-F02:C29544 (NEDD4 Human)
Tags
His and TEV cleavage site
Promoter
T7-lacO
Available Since
Oct. 7, 2015
Availability
Available

Sequence Information

Length
1,016 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 44..62 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
TEV site tobacco etch virus (TEV) protease recog… 74..95 Fwd
DNA Sequence
GAAAACTTGTATTTCCAGGGC
Protein Sequence
ENLYFQG