pT7_circRAJ31_C873U

69932 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant is deficient in circularisation. Under control of a T7 promoter.
Depositor
Alfonso Jaramillo
Article
Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do
Insert
pT7_circRAJ31_C873U
Promoter
T7
Available Since
Feb. 10, 2016
Availability
Available

Sequence Information

Length
820 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

terminator 2
Label Gene Product Position Strand
T7 terminator — — 348..396 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
his operon terminator — — 402..461 Fwd
DNA Sequence
TCCGGCAAAAAAACGGCAAGGTGTCACCACCCTGCCCTTTTTCTTTAAAACCGAAAAGA