pcDNA3-CARD14-D176H

69919 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses mutant FLAG-CARD14 in mammalian cells
Depositor
Francesca Capon
Article
Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.
Insert
CARD14 isoform 3 (CARD14 Human)
Tags
FLAG
Mutation
D176H
Promoter
CMV
Available Since
Feb. 2, 2016
Availability
Available

Sequence Information

Length
1,037 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
FLAG — FLAG(R) epitope tag, followed by an ent… 120..144 Fwd
DNA Sequence
GACTACAAGGACGACGATGACAAG
Protein Sequence
DYKDDDDK
promoter 1
Label Gene Product Position Strand
T7 promoter — — 60..79 Fwd
DNA Sequence
TAATACGACTCACTATAGG