pET23a-MmRPS27a

69559 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Bacterial expression; in vitro transcription/translation (T7)
Depositor
Jorge Eduardo Azevedo
Article
Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103
Insert
RPS27A (RPS27A Mouse, Human)
Mutation
Changed Alanine 133 (human RPS27a) to Glycine (mo…
Promoter
T7
Available Since
Dec. 10, 2015
Availability
Available

Sequence Information

Length
844 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
ubiquitin Homo sapiens UBC human ubiquitin 22..250 Fwd
DNA Sequence
ATGCAGATTTTCGTGAAAACCCTTACGGGGAAGACCATCACCCTCGAGGTTGAACCCTCGGATACGATAGAAAATGTAAAGGCCAAGATCCAGGATAAGGAAGGAATTCCTCCTGATCAGCAGAGACTGATCTTTGCTGGCAAGCAGCTGGAAGATGGACGTACTTTGTCTGACTACAATATTCAAAAGGAGTCTACTCTTCATCTTGTGTTGAGACTTCGTGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
6xHis 6xHis affinity tag 539..557 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator 623..671 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG