pJBL-RNAINS3

69455 Synthetic Bacterial Expression

Plasmid Information

Purpose
RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)
Depositor
Julius Lucks
Article
Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8
Insert
RNA-IN S3
Mutation
mutations for S3 variant (from wt)
Promoter
J23119 and J23119 (sigma 70)
Available Since
Sept. 22, 2015
Availability
Available

Sequence Information

Length
817 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
J23119(SpeI) promoter — — 180..215 Fwd
DNA Sequence
TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT