pBALU9

69324 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOP
Depositor
Oliver Hobert
Article
Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
Insert
gSL2-NLS-GFPint-FgF
Available Since
Sept. 10, 2015
Availability
Available

Sequence Information

Length
1,066 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
SV40 NLS — nuclear localization signal of SV40 (si… 325..346 Fwd
DNA Sequence
CCAAAGAAGAAACGCAAAGTA
Protein Sequence
PKKKRKV
promoter 1
Label Gene Product Position Strand
EM7 promoter — — 793..841 Fwd
DNA Sequence
GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGAC
protein_bind 1
Label Gene Product Position Strand
FRT (minimal) — — 756..790 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC