pBALU3 — PlasmidDB

Plasmid Information

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of cfp

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

CFPint-FgF

Available Since

Sept. 30, 2015

Availability

Available

Sequence Information

Length

834 bp

Topology

linear

Molecule Type

genomic DNA

View on AddGene.org

Features

promoter 1

Label	Gene	Product	Position	Strand
EM7 promoter	—	—	458..506	Fwd
DNA Sequence GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGAC

protein_bind 1

Label	Gene	Product	Position	Strand
FRT (minimal)	—	—	421..455	Rev
DNA Sequence GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC