pBALU2

69317 Synthetic Bacterial Expression

Plasmid Information

Purpose
Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of yfp
Depositor
Oliver Hobert
Article
Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
Insert
YFPint-FgF
Available Since
Sept. 10, 2015
Availability
Available

Sequence Information

Length
1,072 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
EM7 promoter — — 504..552 Fwd
DNA Sequence
GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGAC
protein_bind 1
Label Gene Product Position Strand
FRT (minimal) — — 467..501 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC