pGEX-GST-CHD5(Chromos)-FRAG

68875 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
IPTG inducible bacterial expression of CHD5 fragment
Depositor
Adrian Bracken
Article
Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:
Insert
CHD5 (CHD5 Human)
Tags
GST
Mutation
only the two chromodomains of CHD5
Promoter
tac
Available Since
Oct. 8, 2015
Availability
Available

Sequence Information

Length
1,016 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
HRV 3C site — recognition and cleavage site for human… 13..37 Fwd
DNA Sequence
CTGGAAGTTCTGTTCCAGGGGCCC
Protein Sequence
LEVLFQGP
protein_bind 2
Label Gene Product Position Strand
attB1 mutant version of attB — 46..71 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
attB2 mutant version of attB — 733..758 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT