pTriEx4-Cdc42-2G

68814 Synthetic Bacterial Expression

Plasmid Information

Purpose
FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)
Depositor
Olivier Pertz
Article
Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10
Insert
Cdc42 activation reporter
Tags
His and Myc
Promoter
CMV
Available Since
March 1, 2016
Availability
Available

Sequence Information

Length
763 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 117..135 Fwd
DNA Sequence
CACCATCACCACCATCAC
Protein Sequence
HHHHHH
Myc — Myc (human c-Myc proto-oncogene) epitop… 144..174 Fwd
DNA Sequence
GAGCAGAAACTAATATCAGAGGAGGATCTT
Protein Sequence
EQKLISEEDL