pGEX-2T FRB Y2105A

68726 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR
Depositor
Jie Chen
Article
Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
Insert
FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
Tags
GST-tagged
Mutation
FRB domain only aa2015-2114; Y2105A
Available Since
April 19, 2016
Availability
Available

Sequence Information

Length
830 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
FRB — FKBP-rapamycin binding domain of human … 29..308 Fwd
DNA Sequence
ATCCTCTGGCATGAGATGTGGCATGAAGGCCTGGAAGAGGCATCTCGTTTGTACTTTGGGGAAAGGAACGTGAAAGGCATGTTTGAGGTGCTGGAGCCCTTGCATGCTATGATGGAACGGGGCCCCCAGACTCTGAAGGAAACATCCTTTAATCAGGCCTATGGTCGAGATTTAATGGAGGCCCAAGAGTGGTGCAGGAAGTACATGAAATCAGGGAATGTCAAGGACCTCACCCAAGCCTGGGACCTCTATGCTCATGTGTTCCGACGAATCTCAAAG
Protein Sequence
ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYAHVFRRISK
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 624..729 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT