V96 ELP

68392 Synthetic Bacterial Expression

Plasmid Information

Purpose
Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue
Depositor
Andrew MacKay
Article
Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:
Insert
V96 ELP
Promoter
T7
Available Since
Oct. 1, 2015
Availability
Available

Sequence Information

Length
621 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 14..32 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
HSV tag — HSV (herpes simplex virus) epitope tag 38..71 Rev
DNA Sequence
CAGCCAGAACTCGCCCCGGAAGACCCCGAGGAT
Protein Sequence
QPELAPEDPED