MBP-MEK1 S222TAG

68302 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression
Depositor
Jesse Rinehart
Article
Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1
Insert
MBP-MEK1 S222TAG (MAP2K1 Human, Synthetic)
Tags
His and MBP
Available Since
Sept. 10, 2015
Availability
Available

Sequence Information

Length
837 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
Factor Xa site Factor Xa recognition and cleavage site 28..40 Fwd
DNA Sequence
ATCGAGGGAAGG
Protein Sequence
IEGR
TEV site tobacco etch virus (TEV) protease recog… 40..61 Fwd
DNA Sequence
GAAAATCTGTATTTTCAAGGT
Protein Sequence
ENLYFQG