p415-PtrpC-Cas9-TtrpC-CYC1t

Plasmid Information

Purpose

Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

Depositor

Christian Hong

Article

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

humanized Cas9

Available Since

Oct. 29, 2015

Availability

Available

Length

879 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
SV40 NLS	—	nuclear localization signal of SV40 (si…	857..878	Rev
DNA Sequence CCCAAGAAGAAGAGGAAGGTG Protein Sequence PKKKRKV

promoter 1

Label	Gene	Product	Position	Strand
T7 promoter	—	—	6..25	Fwd
DNA Sequence TAATACGACTCACTATAGG

terminator 1

Label	Gene	Product	Position	Strand
trpC terminator	trpC	—	118..686	Rev
DNA Sequence TGATTTAATAGCTCCATGTCAACAAGAATAAAACGCGTTTCGGGTTTACCTCTTCCAGATACAGCTCATCTGCAATGCATTAATGCATTGGACCTCGCAACCCTAGTACGCCCTTCAGGCTCCGGCGAAGCAGAAGAATAGCTTAGCAGAGTCTATTTTCATTTTCGGGAGACGAGATCAAGCAGATCAACGGTCGTCAAGAGACCTACGAGACTGAGGAATCCGCTCTTGGCTCCACGCGACTATATATTTGTCTCTAATTGTACTTTGACATGCTCCTCTTCTTTACTCTGATAGCTTGACTATGAAAATTCCGTCACCAGCCCCTGGGTTCGCAAAGATAATTGCACTGTTTCTTCCTTGAACTCTCAAGCCTACAGGACACACATTCATCGTAGGTATAAACCTCGAAAATCATTCCTACTAAGATGGGTATACAATAGTAACCATGGTTGCCTAGTGAATGCTCCGTAACACCCAATACGCCGGCCGAAACTTTTTTACAACTCTCCTATGAGTCGTTTACCCAGAATGCACAGGTACACTTGTTTAGAGGTAATCCTTCTTT