pMAL-PIAL2M-sim1

67908 Arabidopsis thaliana Bacterial Expression

Plasmid Information

Purpose
Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.
Depositor
Andreas Bachmair
Article
Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi
Insert
PIAL2 (AT5G41580 Mustard Weed)
Tags
MBP
Mutation
E281-A496; VEDL 425-428 AAA
Promoter
P-lac
Available Since
Aug. 5, 2015
Availability
Available

Sequence Information

Length
830 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
Factor Xa site Factor Xa recognition and cleavage site 17..29 Fwd
DNA Sequence
ATCGAGGGAAGG
Protein Sequence
IEGR