pET28-MRPP1

67862 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)
Depositor
Walter Rossmanith
Article
Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10
Insert
MRPP1 (TRMT10C Human)
Tags
His
Mutation
mitoch. form (contains aa 40-403)
Promoter
T7
Available Since
Aug. 14, 2015
Availability
Available

Sequence Information

Length
1,142 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 59..77 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 86..104 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS