pET-scFv-T

67843 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.
Depositor
Joanna Bereta
Article
Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24
Insert
antibody 1D8 light kappa chain
Tags
His-tag
Promoter
T7 promoter
Available Since
Aug. 6, 2015
Availability
Available

Sequence Information

Length
905 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 780..798 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
6xHis — 6xHis affinity tag 825..843 Rev
DNA Sequence
CATCATCATCACCATCAC
Protein Sequence
HHHHHH
rep_origin 1
Label Gene Product Position Strand
f1 ori — — 174..630 Rev
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 666..714 Rev
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG