pRAV23 — PlasmidDB

Plasmid Information

Purpose

Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Promoter

T7 RNA polymerase

Available Since

Aug. 3, 2015

Availability

Available

Length

1,090 bp

Topology

linear

Molecule Type

genomic DNA

promoter 2

Label	Gene	Product	Position	Strand
T7 promoter	—	—	5..24	Fwd
DNA Sequence TAATACGACTCACTATAGG
lac promoter	—	—	408..439	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	384..401	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	453..475	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	294..342	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG