pME-6MTzactMap2k2b

67710 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Middle entry clone
Depositor
Nathan Lawson
Article
Shin et al Development. 2016 Oct 15;143(20):3785-37
Insert
activated Map2k2b (map2k2b Zebrafish)
Mutation
S221D, S225D
Promoter
none
Available Since
Aug. 7, 2015
Availability
Available

Sequence Information

Length
1,152 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 6
Label Gene Product Position Strand
Myc — Myc (human c-Myc proto-oncogene) epitop… 155..185 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 194..224 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 233..263 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 272..302 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 311..341 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc — Myc (human c-Myc proto-oncogene) epitop… 374..404 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
protein_bind 1
Label Gene Product Position Strand
attL1 mutant version of attL — 11..111 Fwd
DNA Sequence
CAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCT