pT040 — PlasmidDB

Plasmid Information

Purpose

URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Insert

single guide RNA expression cassette

Promoter

pSNR52

Available Since

Aug. 31, 2015

Availability

Available

Length

863 bp

Topology

linear

Molecule Type

genomic DNA

promoter 3

Label	Gene	Product	Position	Strand
SNR52 promoter	—	—	32..301	Fwd
DNA Sequence TCTTTGAAAAGATAATGTATGATTATGCTTTCACTCATATTTATACAGAAACTTGATGTTTTCTTTCGAGTATATACAAGGTGATTACATGTACGTTTGAAGTACAACTCTAGATTTTGTAGTGCCCTCTTGGGCTAGCGGTAAAGGTGCGCATTTTTTCACACCCTACAATGTTCTGTTCAAAAGATTTTGGTCAAACGCTGTAGAAGTGAAAGTTGGTGCGCATGTTTCGGCGTTCGAAACTTCTCCGCAGTGAAAGATAAATGATC
T3 promoter	—	—	587..606	Rev
DNA Sequence AATTAACCCTCACTAAAGG
lac promoter	—	—	674..705	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG

protein_bind 2

Label	Gene	Product	Position	Strand
lac operator	—	—	650..667	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	719..741	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA

terminator 1

Label	Gene	Product	Position	Strand
SUP4 terminator	—	—	382..402	Fwd
DNA Sequence TTTTTTTGTTTTTTATGTCT