pDEST17-6xHis-ARL2 — PlasmidDB

Plasmid Information

Purpose

Expression of hArl2 with N-term His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Length

910 bp

Topology

linear

Molecule Type

genomic DNA

CDS 1

Label	Gene	Product	Position	Strand
6xHis	—	6xHis affinity tag	41..59	Fwd
DNA Sequence CATCACCATCACCATCAC Protein Sequence HHHHHH

protein_bind 2

Label	Gene	Product	Position	Strand
attB1	mutant version of attB	—	68..93	Fwd
DNA Sequence ACAAGTTTGTACAAAAAAGCAGGCT
attB2	mutant version of attB	—	669..694	Rev
DNA Sequence ACCACTTTGTACAAGAAAGCTGGGT

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	760..808	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG