pET-DEST42-SARA-V5-His6

67431 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of hSara with C-term V5 and His6 tag in E. coli
Depositor
Richard Kahn
Article
Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.
Insert
SARA (SAR1B Human)
Tags
His and V5
Promoter
T7/lacO
Available Since
Oct. 5, 2015
Availability
Available

Sequence Information

Length
931 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
V5 tag — epitope tag from simian virus 5 720..762 Fwd
DNA Sequence
GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG
Protein Sequence
GKPIPNPLLGLDST
6xHis — 6xHis affinity tag 771..789 Fwd
DNA Sequence
CATCATCACCATCACCAT
Protein Sequence
HHHHHH
protein_bind 2
Label Gene Product Position Strand
attB1 mutant version of attB — 39..64 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
attB2 mutant version of attB — 676..701 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 859..907 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACCGGGTCTTGAGGGGTTTTTT