pET-DEST42-SAR1-V5-His6

67430 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of hSar1 with C-term V5 and His6 tag in E. coli
Depositor
Richard Kahn
Article
Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.
Insert
SAR1 (SAR1A Human)
Tags
His and V5
Promoter
T7/lacO
Available Since
Oct. 5, 2015
Availability
Available

Sequence Information

Length
880 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
V5 tag — epitope tag from simian virus 5 721..763 Fwd
DNA Sequence
GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG
Protein Sequence
GKPIPNPLLGLDST
6xHis — 6xHis affinity tag 772..790 Fwd
DNA Sequence
CATCATCACCATCACCAT
Protein Sequence
HHHHHH
protein_bind 2
Label Gene Product Position Strand
attB1 mutant version of attB — 40..65 Fwd
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
attB2 mutant version of attB — 677..702 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT