pET-DEST42-ARL14-V5-His6

67427 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of hArl14 with C-term V5 and His6 tag in E. coli
Depositor
Richard Kahn
Article
Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.
Insert
ARL14 (ARL14 Human)
Tags
His and V5
Mutation
bp 172 C to T; aa L58F
Promoter
T7/lacO
Available Since
Oct. 5, 2015
Availability
Available

Sequence Information

Length
941 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 28..46 Rev
DNA Sequence
CATCATCACCATCACCAT
Protein Sequence
HHHHHH
V5 tag — epitope tag from simian virus 5 55..97 Rev
DNA Sequence
GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG
Protein Sequence
GKPIPNPLLGLDST
promoter 1
Label Gene Product Position Strand
T7 promoter — — 825..844 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 3
Label Gene Product Position Strand
attB2 mutant version of attB — 116..141 Fwd
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT
attB1 mutant version of attB — 735..760 Rev
DNA Sequence
ACAAGTTTGTACAAAAAAGCAGGCT
lac operator — — 800..825 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC