pDEST14-ARL1 — PlasmidDB

Plasmid Information

Purpose

Expression of native hArl1 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Mutation

bp 231 C to T; silent mutation

Promoter

Available Since

Oct. 5, 2015

Availability

Available

Length

826 bp

Topology

linear

Molecule Type

genomic DNA

protein_bind 1

Label	Gene	Product	Position	Strand
attB2	mutant version of attB	—	579..604	Rev
DNA Sequence ACCACTTTGTACAAGAAAGCTGGGT

terminator 1

Label	Gene	Product	Position	Strand
T7 terminator	—	—	670..718	Fwd
DNA Sequence CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG