pDEST14-ARF5

67372 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expression of native hArf5 in E. coli
Depositor
Richard Kahn
Article
Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.
Insert
ARF5 (ARF5 Human)
Mutation
bp 477 T to C; silent mutation
Promoter
T7
Available Since
Oct. 5, 2015
Availability
Available

Sequence Information

Length
896 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

protein_bind 1
Label Gene Product Position Strand
attB2 mutant version of attB — 576..601 Rev
DNA Sequence
ACCACTTTGTACAAGAAAGCTGGGT
terminator 1
Label Gene Product Position Strand
T7 terminator — — 667..715 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG