pET-24a-ELP[V-150]

67015 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses ELP[V-150] in E. coli
Depositor
Ashutosh Chilkoti
Article
Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat
Insert
ELP[V-150]
Tags
His6
Promoter
T7
Available Since
Jan. 5, 2016
Availability
Available

Sequence Information

Length
453 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 148..166 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 57..76 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 76..101 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC