pET-24a-ELP[V-30]

67014 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses ELP[V-30] in E. coli
Depositor
Ashutosh Chilkoti
Article
Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat
Insert
ELP[V-30]
Tags
His6
Promoter
T7
Available Since
Jan. 5, 2016
Availability
Available

Sequence Information

Length
728 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 39..57 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
6xHis — 6xHis affinity tag 583..601 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 667..715 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG