pMAL-c5X-poly(alanylglycine)

67003 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses malE-poly(alanylglycine) in E. coli
Depositor
Ashutosh Chilkoti
Article
Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat
Insert
malE-poly(alanylglycine)
Tags
His6 and malE-Factor Xa
Promoter
tac
Available Since
Jan. 5, 2016
Availability
Available

Sequence Information

Length
810 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 518..536 Rev
DNA Sequence
CATCATCACCACCATCAT
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 179..270 Rev
DNA Sequence
TTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
terminator 2
Label Gene Product Position Strand
rrnB T2 terminator — — 297..325 Rev
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT
rrnB T1 terminator Escherichia coli rrnB — 416..503 Rev
DNA Sequence
CAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT