pOPINK-TRABID (NZF1-3, aa 1-178)

66185 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Expresses TRABID NZF1-3 (aa 1-178) in E. coli
Depositor
David Komander
Article
Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:
Insert
TRABID (ZRANB1 Human)
Tags
His6-GST
Mutation
only codes for amino acids 1-178 of TRABID
Promoter
T7
Available Since
July 17, 2015
Availability
Available

Sequence Information

Length
726 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
6xHis 6xHis affinity tag 15..33 Rev
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
HRV 3C site recognition and cleavage site for human… 590..614 Rev
DNA Sequence
CTGGAAGTTCTGTTTCAGGGCCCG
Protein Sequence
LEVLFQGP