pET15b+PCNA3 R112C/T180C

66182 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3
Depositor
Teruyuki Nagamune
Article
Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-
Insert
DNA polymerase sliding clamp 3
Tags
His6 tag
Mutation
R112C, T180C
Promoter
T7 promoter
Available Since
June 30, 2015
Availability
Available

Sequence Information

Length
927 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 805..823 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 832..850 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
protein_bind 1
Label Gene Product Position Strand
lac operator 909..926 Fwd
DNA Sequence
TTGTTATCCGCTCACAA