pET15b+PCNA3 T180C

66181 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses the T180C mutant of Sulfolobus solfataricus PCNA3
Depositor
Teruyuki Nagamune
Article
Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-
Insert
DNA polymerase sliding clamp 3
Tags
His6 tag
Mutation
T180C
Promoter
T7 promoter
Available Since
June 30, 2015
Availability
Available

Sequence Information

Length
881 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 2
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 806..824 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis 6xHis affinity tag 833..851 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH