pET15b+PCNA3 R112C

66180 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses the R112C mutant of Sulfolobus solfataricus PCNA3
Depositor
Teruyuki Nagamune
Article
Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-
Insert
DNA polymerase sliding clamp 3
Tags
His6 tag
Mutation
R112C
Promoter
T7 promoter
Available Since
June 30, 2015
Availability
Available

Sequence Information

Length
501 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 1
Label Gene Product Position Strand
thrombin site thrombin recognition and cleavage site 5..23 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS