pET15b+PCNA2 E105C/L171C

66178 Synthetic Bacterial Expression

Plasmid Information

Purpose
Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2
Depositor
Teruyuki Nagamune
Article
Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-
Insert
DNA polymerase sliding clamp 2
Tags
His6 tag
Mutation
E105C, L171C
Promoter
T7 promoter
Available Since
June 30, 2015
Availability
Available

Sequence Information

Length
979 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
thrombin site — thrombin recognition and cleavage site 766..784 Rev
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
6xHis — 6xHis affinity tag 793..811 Rev
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
promoter 1
Label Gene Product Position Strand
T7 promoter — — 891..910 Rev
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 866..891 Fwd
DNA Sequence
GGAATTGTTATCCGCTCACAATTCC