213-petDuet1-GBD-LUMP--cdc42

65973 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
Dual expression of GBD-LUMP and cdc42
Depositor
Gerard Marriott
Article
Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p
Tags
GBD (GTPase binding domain from ACK1; residues 44…
Mutation
constitutively active Cdc42 (Q61L)
Promoter
T7
Available Since
July 13, 2015
Availability
Available

Sequence Information

Length
876 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
T7 promoter — — 48..67 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 67..92 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC