pT7-gfap-sgRNA

65566 Danio rerio Bacterial Expression

Plasmid Information

Purpose
in vitro trancription of sgRNA targeting the zebrafish gfap locus
Depositor
Jiu-lin Du
Article
Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20
Insert
gfap sgRNA (gfap Zebrafish)
Promoter
T7
Available Since
June 8, 2015
Availability
Available

Sequence Information

Length
1,095 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 1
Label Gene Product Position Strand
lac promoter — — 385..416 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 349..371 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 423..440 Fwd
DNA Sequence
TTGTGAGCGGATAACAA