pT7-th-sgRNA

65565 Danio rerio Bacterial Expression

Plasmid Information

Purpose
in vitro trancription of sgRNA targeting the zebrafish th locus
Depositor
Jiu-lin Du
Article
Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20
Insert
th sgRNA (th Zebrafish)
Promoter
T7
Available Since
June 8, 2015
Availability
Available

Sequence Information

Length
1,097 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

promoter 2
Label Gene Product Position Strand
lac promoter — — 383..414 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
T7 promoter — — 514..533 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 347..369 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 421..438 Fwd
DNA Sequence
TTGTGAGCGGATAACAA