th-P2A-EGFP donor

65562 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Intron targeting-mediated EGFP knockin at the zebrafish th locus.
Depositor
Jiu-lin Du
Article
Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20
Insert
th-P2A-EGFP (th Zebrafish)
Promoter
none
Available Since
June 3, 2015
Availability
Available

Sequence Information

Length
963 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
P2A — 2A peptide from porcine teschovirus-1 p… 451..508 Rev
DNA Sequence
GCTACTAATTTCTCCTTGCTTAAGCAAGCTGGTGATGTTGAAGAAAATCCTGGTCCT
Protein Sequence
ATNFSLLKQAGDVEENPGP